A TaqMan-based multiplex real-time PCR assay for the simultaneous detection of Wuchereria bancrofti and Brugia malayi.
Identifieur interne : 003953 ( Main/Exploration ); précédent : 003952; suivant : 003954A TaqMan-based multiplex real-time PCR assay for the simultaneous detection of Wuchereria bancrofti and Brugia malayi.
Auteurs : N. Pilotte [États-Unis] ; M. Torres ; F R Tomaino ; S J Laney ; S A WilliamsSource :
- Molecular and biochemical parasitology [ 1872-9428 ] ; 2013.
Descripteurs français
- KwdFr :
- ADN des helminthes (génétique), ADN des helminthes (isolement et purification), Animaux, Brugia malayi (génétique), Brugia malayi (isolement et purification), Culicidae (parasitologie), Filariose lymphatique (diagnostic), Filariose lymphatique (parasitologie), Humains, Parasitologie (), Parasitologie (économie), Réaction de polymérisation en chaine en temps réel (), Réaction de polymérisation en chaine en temps réel (économie), Réaction de polymérisation en chaine multiplex (), Réaction de polymérisation en chaine multiplex (économie), Sang (parasitologie), Sensibilité et spécificité, Techniques de diagnostic moléculaire (), Techniques de diagnostic moléculaire (économie), Wuchereria bancrofti (génétique), Wuchereria bancrofti (isolement et purification).
- MESH :
- diagnostic : Filariose lymphatique.
- génétique : ADN des helminthes, Brugia malayi, Wuchereria bancrofti.
- isolement et purification : ADN des helminthes, Brugia malayi, Wuchereria bancrofti.
- parasitologie : Culicidae, Filariose lymphatique, Sang.
- économie : Parasitologie, Réaction de polymérisation en chaine en temps réel, Réaction de polymérisation en chaine multiplex, Techniques de diagnostic moléculaire.
- Animaux, Humains, Parasitologie, Réaction de polymérisation en chaine en temps réel, Réaction de polymérisation en chaine multiplex, Sensibilité et spécificité, Techniques de diagnostic moléculaire.
English descriptors
- KwdEn :
- Animals, Blood (parasitology), Brugia malayi (genetics), Brugia malayi (isolation & purification), Culicidae (parasitology), DNA, Helminth (genetics), DNA, Helminth (isolation & purification), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (parasitology), Humans, Molecular Diagnostic Techniques (economics), Molecular Diagnostic Techniques (methods), Multiplex Polymerase Chain Reaction (economics), Multiplex Polymerase Chain Reaction (methods), Parasitology (economics), Parasitology (methods), Real-Time Polymerase Chain Reaction (economics), Real-Time Polymerase Chain Reaction (methods), Sensitivity and Specificity, Wuchereria bancrofti (genetics), Wuchereria bancrofti (isolation & purification).
- MESH :
- chemical , genetics : DNA, Helminth.
- diagnosis : Elephantiasis, Filarial.
- economics : Molecular Diagnostic Techniques, Multiplex Polymerase Chain Reaction, Parasitology, Real-Time Polymerase Chain Reaction.
- genetics : Brugia malayi, Wuchereria bancrofti.
- isolation & purification : Brugia malayi, DNA, Helminth, Wuchereria bancrofti.
- methods : Molecular Diagnostic Techniques, Multiplex Polymerase Chain Reaction, Parasitology, Real-Time Polymerase Chain Reaction.
- parasitology : Blood, Culicidae, Elephantiasis, Filarial.
- Animals, Humans, Sensitivity and Specificity.
Abstract
With the Global Program for the Elimination of Lymphatic Filariasis continuing to make strides towards disease eradication, many locations endemic for the causative parasites of lymphatic filariasis are realizing a substantial decrease in levels of infection and rates of disease transmission. However, with measures of disease continuing to decline, the need for time-saving and economical molecular diagnostic assays capable of detecting low levels of parasite presence is increasing. This need is greatest in locations co-endemic for both Wuchereria bancrofti and Brugia parasites because testing for both causative agents individually results in significant increases in labor and reagent costs. Here we describe a multiplex, TaqMan-based, real-time PCR assay capable of simultaneously detecting W. bancrofti and Brugia malayi DNA extracted from human bloodspots or vector mosquito pools. With comparable sensitivity to established singleplex assays, this assay provides significant cost and labor savings for disease monitoring efforts in co-endemic locations.
DOI: 10.1016/j.molbiopara.2013.05.001
PubMed: 23669148
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001B52
- to stream PubMed, to step Curation: 001B52
- to stream PubMed, to step Checkpoint: 001B52
- to stream Ncbi, to step Merge: 005910
- to stream Ncbi, to step Curation: 005910
- to stream Ncbi, to step Checkpoint: 005910
- to stream Main, to step Merge: 003962
- to stream Main, to step Curation: 003953
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A TaqMan-based multiplex real-time PCR assay for the simultaneous detection of Wuchereria bancrofti and Brugia malayi.</title><author><name sortKey="Pilotte, N" sort="Pilotte, N" uniqKey="Pilotte N" first="N" last="Pilotte">N. Pilotte</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biological Sciences, Smith College, Ford Hall, 100 Green Street, Northampton, MA 01063, USA. npilotte@smith.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological Sciences, Smith College, Ford Hall, 100 Green Street, Northampton, MA 01063</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Torres, M" sort="Torres, M" uniqKey="Torres M" first="M" last="Torres">M. Torres</name></author><author><name sortKey="Tomaino, F R" sort="Tomaino, F R" uniqKey="Tomaino F" first="F R" last="Tomaino">F R Tomaino</name></author><author><name sortKey="Laney, S J" sort="Laney, S J" uniqKey="Laney S" first="S J" last="Laney">S J Laney</name></author><author><name sortKey="Williams, S A" sort="Williams, S A" uniqKey="Williams S" first="S A" last="Williams">S A Williams</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23669148</idno><idno type="pmid">23669148</idno><idno type="doi">10.1016/j.molbiopara.2013.05.001</idno><idno type="wicri:Area/PubMed/Corpus">001B52</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001B52</idno><idno type="wicri:Area/PubMed/Curation">001B52</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001B52</idno><idno type="wicri:Area/PubMed/Checkpoint">001B52</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001B52</idno><idno type="wicri:Area/Ncbi/Merge">005910</idno><idno type="wicri:Area/Ncbi/Curation">005910</idno><idno type="wicri:Area/Ncbi/Checkpoint">005910</idno><idno type="wicri:Area/Main/Merge">003962</idno><idno type="wicri:Area/Main/Curation">003953</idno><idno type="wicri:Area/Main/Exploration">003953</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A TaqMan-based multiplex real-time PCR assay for the simultaneous detection of Wuchereria bancrofti and Brugia malayi.</title><author><name sortKey="Pilotte, N" sort="Pilotte, N" uniqKey="Pilotte N" first="N" last="Pilotte">N. Pilotte</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biological Sciences, Smith College, Ford Hall, 100 Green Street, Northampton, MA 01063, USA. npilotte@smith.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological Sciences, Smith College, Ford Hall, 100 Green Street, Northampton, MA 01063</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Torres, M" sort="Torres, M" uniqKey="Torres M" first="M" last="Torres">M. Torres</name></author><author><name sortKey="Tomaino, F R" sort="Tomaino, F R" uniqKey="Tomaino F" first="F R" last="Tomaino">F R Tomaino</name></author><author><name sortKey="Laney, S J" sort="Laney, S J" uniqKey="Laney S" first="S J" last="Laney">S J Laney</name></author><author><name sortKey="Williams, S A" sort="Williams, S A" uniqKey="Williams S" first="S A" last="Williams">S A Williams</name></author></analytic><series><title level="j">Molecular and biochemical parasitology</title><idno type="eISSN">1872-9428</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blood (parasitology)</term><term>Brugia malayi (genetics)</term><term>Brugia malayi (isolation & purification)</term><term>Culicidae (parasitology)</term><term>DNA, Helminth (genetics)</term><term>DNA, Helminth (isolation & purification)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Humans</term><term>Molecular Diagnostic Techniques (economics)</term><term>Molecular Diagnostic Techniques (methods)</term><term>Multiplex Polymerase Chain Reaction (economics)</term><term>Multiplex Polymerase Chain Reaction (methods)</term><term>Parasitology (economics)</term><term>Parasitology (methods)</term><term>Real-Time Polymerase Chain Reaction (economics)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des helminthes (génétique)</term><term>ADN des helminthes (isolement et purification)</term><term>Animaux</term><term>Brugia malayi (génétique)</term><term>Brugia malayi (isolement et purification)</term><term>Culicidae (parasitologie)</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (parasitologie)</term><term>Humains</term><term>Parasitologie ()</term><term>Parasitologie (économie)</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Réaction de polymérisation en chaine en temps réel (économie)</term><term>Réaction de polymérisation en chaine multiplex ()</term><term>Réaction de polymérisation en chaine multiplex (économie)</term><term>Sang (parasitologie)</term><term>Sensibilité et spécificité</term><term>Techniques de diagnostic moléculaire ()</term><term>Techniques de diagnostic moléculaire (économie)</term><term>Wuchereria bancrofti (génétique)</term><term>Wuchereria bancrofti (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Helminth</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="economics" xml:lang="en"><term>Molecular Diagnostic Techniques</term><term>Multiplex Polymerase Chain Reaction</term><term>Parasitology</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des helminthes</term><term>Brugia malayi</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Brugia malayi</term><term>DNA, Helminth</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN des helminthes</term><term>Brugia malayi</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term><term>Multiplex Polymerase Chain Reaction</term><term>Parasitology</term><term>Real-Time Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Culicidae</term><term>Filariose lymphatique</term><term>Sang</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Blood</term><term>Culicidae</term><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="économie" xml:lang="fr"><term>Parasitologie</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Réaction de polymérisation en chaine multiplex</term><term>Techniques de diagnostic moléculaire</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Parasitologie</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Réaction de polymérisation en chaine multiplex</term><term>Sensibilité et spécificité</term><term>Techniques de diagnostic moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">With the Global Program for the Elimination of Lymphatic Filariasis continuing to make strides towards disease eradication, many locations endemic for the causative parasites of lymphatic filariasis are realizing a substantial decrease in levels of infection and rates of disease transmission. However, with measures of disease continuing to decline, the need for time-saving and economical molecular diagnostic assays capable of detecting low levels of parasite presence is increasing. This need is greatest in locations co-endemic for both Wuchereria bancrofti and Brugia parasites because testing for both causative agents individually results in significant increases in labor and reagent costs. Here we describe a multiplex, TaqMan-based, real-time PCR assay capable of simultaneously detecting W. bancrofti and Brugia malayi DNA extracted from human bloodspots or vector mosquito pools. With comparable sensitivity to established singleplex assays, this assay provides significant cost and labor savings for disease monitoring efforts in co-endemic locations.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><noCountry><name sortKey="Laney, S J" sort="Laney, S J" uniqKey="Laney S" first="S J" last="Laney">S J Laney</name><name sortKey="Tomaino, F R" sort="Tomaino, F R" uniqKey="Tomaino F" first="F R" last="Tomaino">F R Tomaino</name><name sortKey="Torres, M" sort="Torres, M" uniqKey="Torres M" first="M" last="Torres">M. Torres</name><name sortKey="Williams, S A" sort="Williams, S A" uniqKey="Williams S" first="S A" last="Williams">S A Williams</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Pilotte, N" sort="Pilotte, N" uniqKey="Pilotte N" first="N" last="Pilotte">N. Pilotte</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003953 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003953 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:23669148
|texte= A TaqMan-based multiplex real-time PCR assay for the simultaneous detection of Wuchereria bancrofti and Brugia malayi.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23669148" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |